Qualitative and quantitative identification of various compounds, including cyanotoxins, with HPLC and ELISA techniques. | HPLC Shimadzu Prominence | |
Toxicity assessment of environmental samples with biotests with bacteria, algae, plants or invertebrates. |
MARA (Microbial Assay for toxic Risk Assessment) |
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Spirodela duckweed microbiotest | ||
Genotoxicity assessment on bacteria and human and animal cell cultures |
Ames assay (MPF) |
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Cytokinesis-block micronucleus assay; 8-oxoguanine detection | ||
Primary cells and stable cell line culturing, transfection techniques, slide preparation and staining techniques, including i.a. visualization of cell cytoskeleton, apoptosis detection by the detection of membrane changes |
Phosphatidylserine translocation in cells
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Actin/tubulin immunolocalisation in cells |
Others: spectrophotometric/luminometric/fluorimetric assays on different cell functions/status; assessment of biochemical indices in body fluids; nucleic acid isolation; western blotting.